Document 0788
 DOCN  M94B0788
 TI    Analysis of peripheral lymphocytes of lymphoma patients for daunomycin
       (DNR) retention and cell differentiation antigens (Meeting abstract).
 DT    9412
 AU    Samy TS; Byrnes J; Krishan A; Harrington WJ Jr; Byrnes G; Wellham LL;
       Sauerteig A; Fletcher MA; University of Miami School of Medicine, Miami,
       FL 33136
 SO    Proc Annu Meet Am Assoc Cancer Res; 35:A1846 1994. Unique Identifier :
       AIDSLINE ICDB/94603532
 AB    The lymph node cells and peripheral blood lymphocytes of lymphoma
       patients were analyzed for cellular DNR retention, response to efflux
       blockers and expression of human differentiation antigens. Extensive
       heterogeneity in cellular DNR retention was seen in lymphocytes in 10/66
       patients. Cellular DNR retention could be significantly increased by
       coincubation with 25 uM prochlorperazine (6/10), 10 uM verapamil (2/10)
       or 20 uM dipyridamole (1/10). Analysis for differentiating antigen
       expression showed baseline CD2, CD3, CD8 and CD25 counts significantly
       associated with increased survival of HIV patients while CD4, CD4 subset
       and B cell counts were not. Furthermore, patients with lymphocytes of
       CD8 counts greater than 1000 responded to chemotherapy suggesting that
       CD8 counts may be an important predictor for HIV associated lymphoma.
       These studies underscore the importance of cellular markers in diagnosis
       of lymphoma and suggest the use of appropriate efflux blocker or drug
       retention and a cytokine for CD8 cell enrichment.
 DE    Antigens, CD/*ANALYSIS  Daunorubicin/*PHARMACOKINETICS  Human
       Lymphocyte Subsets/IMMUNOLOGY/*METABOLISM
       Lymphocytes/IMMUNOLOGY/*METABOLISM  Lymphoma/BLOOD/*DRUG
       THERAPY/IMMUNOLOGY/*METABOLISM  Lymphoma, AIDS-Related/BLOOD/*DRUG
       THERAPY/IMMUNOLOGY  Prognosis  MEETING ABSTRACT

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